ABSTRACT
Material and methods: A random sample of 28 large bowel adenomas and 44 carcinomas was studied. Determination of p53 protein was made with an immunohistochemical method using monoclonal antibodies. Patients were followed for a mean of 36 months (range 1 to 100 months). Results: p53 immunostaining was obtained in one adenoma (3.5 percent) and in 18 carcinomas (41 percent, p = 0.01). There were no differences in survival during follow up, between cancer patients that expressed or did not express p53 protein. Conclusions: About half of colorectal tumors have immunohistochemical expression of p53 protein, as published abroad. We did not find a prognostic value for this protein in our sample
Subject(s)
Humans , Male , Female , Adult , Middle Aged , Colorectal Neoplasms/immunology , Tumor Suppressor Protein p53/isolation & purification , Oncogene Proteins/isolation & purification , Immunohistochemistry/methods , Adenocarcinoma/immunology , Adenoma/immunologyABSTRACT
Mediante una técnica inmunohistoquímica se dterminó la sobreexpresión de p53 en 154 muestras de cáncer gástrico obtenidas por endoscopía. Los resultados se correlacionaron con otras variables pronósticas, con el índice proliferativo PCNA como parámetro de proliferación y con el pronóstico de los pacientes. 54 muestras (35,06 porciento) fueron positivas para p53 (p53(+)). No se logró determinar ninguna asociación entre la positividad de p53 y variables como la localización o el tamaño del tumor, el tipo Borrmann, la presencia de metástasis peritoneales o hepáticas o el grado de resecabilidad de las lesiones. En el momento de la cirugía había compromiso linfático en el 72 porciento de los tumores p53(+) pero sólo en el 50 porciento de los p53(-) (p<0.01). La sobrevida general a cinco años fue de 46,93 porciento. La sobrevida de los tumores p53(+) fue de 29,38 porciento versus un 56,68 porciento para los tumores p53(-)(p<0.05). Se concluye que la determinación inmunohistoquímica de p53 en muestras de cáncer gástrico obtenidas por endoscopía se asocia con mayores posibilidades de compromiso linfático, con un peor pronóstico general y con una mayor actividad proliferativa de los tumores pudiendo llegar a ser un importante parámetro en la evaluación preoperatoria de los pacientes en etapas precoces
Subject(s)
Humans , Immunohistochemistry/methods , Biomarkers, Tumor/isolation & purification , Stomach Neoplasms/pathology , Tumor Suppressor Protein p53/isolation & purification , Gastroscopy/statistics & numerical data , PrognosisABSTRACT
The most common liver tumor in USA and most of the western world is metastasic. Primary hepatic tumors can derive from hepatocytes (hepatocarcinoma), biliary ducts (cholangiocarcinoma) and Kupffer's cells (hemangiosarcoma). The most common primary liver tumor in the world is the hepatocarcinoma, this is due to the high prevalence of the lesion in many areas of Africa and Asia, although it is unusual in the western hemisphere. Risk factors for hepatocarcinoma include: HBsAg carrier state, hepatitis C infection, cirrhosis of the liver, alcoholic liver disease, aflatoxin exposure, thorium dioxide, pesticides, anabolic steroids and hemochromatosis. The regenerative nodule can be considered as a preneoplastic lesion. Several tumoral markers have been found helpful in the diagnosis of this tumor: the classic one AFP is present only in 43 percent of the cases, HBsAg in studies in Taiwan in 58 percent of non neoplastic hepatocytes, AAT in 70 percent of the patients. These marquers are expressed in the liver cell in several forms: granulofibrilar occupying most of the cytoplasm and diffuse granular mainly in malignant hepatocytes. Immunoperoxidase stain shows a thick expression of the granular antigen in regenerating cells: this is true for AFP, AAT and HBsAg. The morphology of the tumor is multicentric in cirrhotic livers or focal in normal ones. Characteristically has a notorius tendency to invade the venous system and generally can appear as: a) nodular, b) massive, c) diffuse. The most common histologic pattern is the trabecular-pseudoglandular. The OMS classification of this lesion described 12 different types. In general the prognosis is very poor with the exception of the fibrolamelar type seen in young people that frequently can be resected...